Application

Research CategoryEpigenetics & Nuclear Function

Anti-CDC7 Antibody detects level of CDC7 & has been published & validated for use in CDC7.

Research Sub CategoryCell Cycle, DNA Replication & Repair

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CDC7, also known as CDC7-related kinase or HsCdc7/HuCdc7, and encoded by the gene CDC7/CDC7L1, is a critical kinase needed to phosphorylate critical substrates that regulate the G1/S phase transition and DNA replication. CDC7 is a serine-threonine kinase that forms a complex with either DBF4 or DBF4B proteins leading to the activation of the kinase activity necessary for its function. Additionally, CDC7 phosphorylates and forms a complex with MCM2 and MCM3 proteins which are highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. CDC7 is localized to the localized in the nucleus, and CDC7 is over expressed in many tumors and transformed cell lines.

Immunogen

KLH-conjugated linear peptide corresponding to the protein kinase domain of human CDC7.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in human spleen tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected CDC7 in 10µg of human spleen tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~63 kDa observed